Identification and comparison of protein-protein i

专利名称：Identification and comparison of protein-protein interactions that occur in

populations and identification of inhibitorsof these interactors

发明人：Krishnan Nandabalan,Jonathan Marc

Rothberg

申请号：US10001670申请日：20011115

公开号：US20030119002A1公开日：20030626

摘要：Methods are described for detecting protein-protein interactions, among twopopulations of proteins, each having a complexity of at least 1,000. For example, proteinsare fused either to the DNA-binding domain of a transcriptional activator or to theactivation domain of a transcriptional activator. Two yeast strains, of the opposite matingtype and carrying one type each of the fusion proteins are mated together. Productiveinteractions between the two halves due to protein-protein interactions lead to thereconstitution of the transcriptional activator, which in turn leads to the activation of areporter gene containing a binding site for the DNA-binding domain. This analysis can becarried out for two or more populations of proteins. The differences in the genesencoding the proteins involved in the protein-protein interactions are characterized, thusleading to the identification of specific protein-protein interactions, and the genesencoding the interacting proteins, relevant to a particular tissue, stage or disease.Furthermore, inhibitors that interfere with these protein-protein interactions are

identified by their ability to inactivate a reporter gene. The screening for such inhibitorscan be in a multiplexed format where a set of inhibitors will be screened against a libraryof interactors.

申请人：CURAGEN CORP.

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