Inactivation resistant factor VIII

专利名称：Inactivation resistant factor VIII

发明人：Kaufman, Randal J.,Pipe, Steven W.,Amano,

Kagehiro

申请号：EP06004484.9申请日：19970424公开号：EP17718A2公开日：20070221

摘要：The present invention provides novel purified and isolated nucleic acid

sequences encoding procoagulant-active FVIII proteins. The nucleic acid sequences of thepresent invention encode amino acid sequences corresponding to known human FVIIIsequences, wherein residue Phe309 is mutated. The nucleic acid sequences of the presentinvention also encode amino acid sequences corresponding to known human FVIIIsequences, wherein the APC cleavage sites, Arg336 and Ile562, are mutated. The nucleicacid sequences of the present invention further encode amino acid sequences

corresponding to known human FVIII sequences, wherein the B-domain is deleted, thevon Willebrand factor binding site is deleted, a thrombin cleavage site is mutated and anamino acid sequence spacer is inserted between the A2- and A3-domains. Methods ofproducing the FVIII proteins of the invention, nucleotide sequences encoding suchproteins, pharmaceutical compositions containing the nucleotide sequences or proteins,as well as methods of treating patients suffering from hemophilia, are also provided.

申请人：THE REGENTS OF THE UNIVERSITY OF MICHIGAN

地址：Technology Management Wolverine Tower Office Room 2071, 3003 South StateStreet Ann Arbor, Michigan 48109-1280 US

国籍：US

代理机构：Vossius & Partner

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