专利名称：Assay for the free portion of substances in
biological fluids
发明人：Charlton, John Cecil,Midgley, John
Edward,Wilkins, Terence Arthur
申请号：EP83301463.2申请日：19830316公开号：EP0089806A1公开日：19830928
摘要：A biological sample containing an analyte, partly free and partly bound tonatural binders, is incubated with a labelled specific binder for the analyte and aderivative of the analyte which does not react with the natural binders. Part of thelabelled specific binder binds to the analyte derivative, the proportion depending on thefree analyte concentration in the sample. The proportion is measured and used todetermine the free analyte concentration. For example, the analyte may be thyroxine orcortisol, the labelled specific binder may be an antibody thereto, and the analytederivative may be in the form of a solid matrix. The labelled specific binder should havean affinity for the analyte, expressed as a dissociation constant of the complex of the two,approximately equal to the concentration of free analyte in the sample.
申请人：AMERSHAM INTERNATIONAL plc
地址：White Lion Road Amersham Buckinghamshire, HP7 9LL GB
国籍：GB
代理机构：Pennant, Pyers
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