专利名称：Fluorescent microspheres and methods of
using them
发明人：Sau W. Cheung申请号：US07/434892申请日：19891113公开号：US05132242A公开日：19920721
摘要：Highly fluorescent latex microspheres have a diameter of less than five hundredangstroms and have more than five thousand fluorescent markers per sphere. Themicrospheres are prepared by reacting an acrylic latex bead with a diamine and afluorescent amine at elevated pH to attach a spacer arm and a marker, respectively, tothe bead by transacylation of ester terminations on the surface of the bead. A proteinsuch as avidin or an immunoglobulin may then be conjugated to the spacer arm. A singlefluorescent microsphere is visible using standard fluorescent microscopy. Therefore themicrospheres may be utilitzed not only to visualize cell surface antigens but also DNAencoding for single genes, by means of a biotinylated DNA probe.
申请人：CHEUNG; SAU W.
代理机构：Polster, Lieder, Woodruff and Lucchesi
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